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Product Name
PAX6 antibody
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Description
PAX6 Rabbit Polyclonal antibody. Positive FC detected in SH-SY5Y cells. Positive IF detected in HeLa cells. Positive IHC detected in human brain tissue, human pancreas tissue. Positive WB detected in Y79 cells, human stomach tissue. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, IHC, WB, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AN2 antibody; Aniridia type II protein antibody; D11S812E antibody; MGDA antibody; OCulorhombin antibody; paired box 6 antibody; Paired box protein Pax 6 antibody; PAX6 antibody; WAGR antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAX6 recombinant protein (Accession Number: NM_001368891). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:113607(PAX6 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113607(PAX6 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113607(PAX6 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:113607(PAX6 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 SH-SY5Y cells were stained with 0.2ug PAX6 antibody (Catalog No:113607, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.
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References
- Zhao X, Wu J, Zheng M, Gao F, Ju G. Specification and maintenance of oligodendrocyte precursor cells from neural progenitor cells: involvement of microRNA-7a. Molecular biology of the cell. 23(15):2867-78. 2012.
- Kreitzer FR, Salomonis N, Sheehan A. A robust method to derive functional neural crest cells from human pluripotent stem cells. American journal of stem cells. 2(2):119-31. 2013.
- Hou C, Ding L, Zhang J. Abnormal cerebellar development and Purkinje cell defects in Lgl1-Pax2 conditional knockout mice. Developmental biology. 395(1):167-81. 2014.
- Zhang C, Ge X, Liu Q, Jiang M, Li MW, Li H. MicroRNA-mediated non-cell-autonomous regulation of cortical radial glial transformation revealed by a Dicer1 knockout mouse model. Glia. 63(5):860-76. 2015.
- Cipriani S, Nardelli J, Verney C. Dynamic Expression Patterns of Progenitor and Pyramidal Neuron Layer Markers in the Developing Human Hippocampus. Cerebral cortex (New York, N.Y. : 1991). 26(3):1255-71. 2016.
- Pan X, Chang X, Leung C. PAK1 regulates cortical development via promoting neuronal migration and progenitor cell proliferation. Molecular brain. 8:36. 2015.
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