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Product Name
PAX3 Polyclonal Antibody
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Description
Polyclonal antibody to PAX3
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PAX3 antibody; CDHS antibody; HUP2 antibody; WS1 antibody; WS3 antibody; paired box 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human PAX3 (NP_039230.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PAX3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PAX3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - PAX3 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using PAX3 antibody . Blue: DAPI for nuclear staining.
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Background
Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"