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Product Name
PARP9 antibody
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Description
PARP9 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in HeLa cells, K-562 cells, MCF7 cells, rat skeletal muscle tissue. Positive IHC detected in human spleen tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human lymphoma tissue, human ovary tissue, human skin tissue, human testis tissue, human tonsillitis tissue. Observed molecular weight by Western-blot: 88kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
B aggressive lymphoma protein antibody; BAL antibody; BAL1 antibody; DKFZp666B0810 antibody; DKFZp686M15238 antibody; FLJ26637 antibody; FLJ35310 antibody; FLJ41418 antibody; FLJ43593 antibody; PARP 9 antibody; PARP9 antibody; Poly [ADP ribose] polymerase 9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PARP9 recombinant protein (Accession Number: NM_001387875). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113596(PARP9 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human spleen using Catalog No:113596(PARP9 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:113596(PARP9 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PARP9 (IP:Catalog No:113596, 4ug; Detection:Catalog No:113596 1:500) with MCF-7 cells lysate 4000ug.
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Background
Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by interferon gamma (IFNγ), inducing the transcription of IFNγ-controlled genes.
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