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Product Name
PARL Polyclonal Antibody
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Description
Polyclonal antibody to PARL
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
PARL antibody; PRO2207 antibody; PSARL antibody; PSARL1 antibody; PSENIP2 antibody; RHBDS1 antibody; presenilin associated rhomboid like antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PARL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PARL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - PARL Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PARL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"