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Product Name
PAPOLG antibody
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Description
PAPOLG Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in HeLa cells, MCF-7 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 83 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ11805 antibody; FLJ12972 antibody; FLJ13482 antibody; FLJ14187 antibody; Neo PAP antibody; Neo poly(A) polymerase antibody; PAP gamma antibody; PAP2 antibody; PAPG antibody; PAPOLG antibody; poly(A) polymerase gamma antibody; SRP RNA 3 adenylating enzyme antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAPOLG recombinant protein (Accession Number: NM_022894). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113577(PAPOLG Antibody) at dilution of 1:1000
IP Result of anti-PAPOLG (IP:Catalog No:113577, 4ug; Detection:Catalog No:113577 1:1000) with HeLa cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113577(PAPOLG Antibody) at dilution of 1:50 (under 40x lens)
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Background
PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.
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