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Product Name
PANK2 antibody
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Description
PANK2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue, human liver tissue. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, HepG2 cells, human brain tissue, MCF7 cells, mouse brain tissue, mouse lung tissue. Observed molecular weight by Western-blot: 59 kDa,48 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C20orf48 antibody; FLJ17232 antibody; HARP antibody; hPanK2 antibody; HSS antibody; NBIA1 antibody; PANK2 antibody; pantothenate kinase 2 antibody; Pantothenic acid kinase 2 antibody; PKAN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PANK2 recombinant protein (Accession Number: BC009421). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:10-1:100
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113570(PANK2 antibody) at dilution of 1:100
IP Result of anti-PANK2 (IP:Catalog No:113570, 3ug; Detection:Catalog No:113570 1:800) with mouse brain tissue lysate 9500ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113570(PANK2 antibody) at dilution of 1:10 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:113570(PANK2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.
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