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Product Name
PAN2 antibody
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Description
PAN2 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human brain tissue, human kidney tissue, human liver tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive IF detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, HepG2 cells. Observed molecular weight by Western-blot: 127 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ39360 antibody; hPAN2 antibody; KIAA0710 antibody; PAN2 antibody; USP52 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAN2 recombinant protein (Accession Number: NM_001394700). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113567(PAN2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human heart using Catalog No:113567(PAN2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113567(PAN2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:113567(PAN2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PAN2 represents PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
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References
- Jonas S, Christie M, Peter D. An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay. Nature structural & molecular biology. 21(7):599-608. 2014.
- Hu Y, Yin KL, Ma X, Xia HF. Anti-PABPC1 co-immunoprecipitation for examining the miRNAs directly targeting the 3'-UTR of EED mRNA. PloS one. 9(8):e103695. 2014.
- Ma J, Fukuda Y, Schultz RM. Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation. Biology of reproduction. 93(2):48. 2015.
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