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Product Name
Pan DiMethyl-lysine pAb
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Description
Polyclonal antibody to Pan DiMethyl-lysine
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat, Other (Wide Range)
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Alternative names
antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence containing dimethylated K.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100 -
Validations
Western blot - Pan DiMethyl-lysine pAb
Western blot analysis of extracts of various cell lines, using pan-Kme2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Dot Blot - Pan DiMethyl-lysine pAb
Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody at 1:1000 dilution.
Immunofluorescence - Pan DiMethyl-lysine pAb
Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Pan DiMethyl-lysine pAb
Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Pan DiMethyl-lysine pAb
Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"