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Product Name
Palladin-Specific antibody
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Description
Palladin-Specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 120kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CGI 151 antibody; FLJ22190 antibody; FLJ38193 antibody; FLJ39139 antibody; KIAA0992 antibody; palladin antibody; PALLD antibody; PNCA1 antibody; Sarcoma antigen NY SAR 77 antibody; SIH002 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_016081). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113563(PALLD,palladin-Specific antibody) at dilution of 1:200
IP Result of anti-PALLD,palladin-Specific (IP:Catalog No:113563, 4ug; Detection:Catalog No:113563 1:200) with HeLa cells lysate 1500ug.
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Background
Palladin is a widely expressed protein found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin. It is a cytoskeletal protein required for organization of normal actin cytoskeleton. It functions in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. This antibody, generated against N-terminal peptide of palladin, recognizes 200 kD, 140 kD, and 65 kD isoforms of this protein.
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