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Product Name
PAK6 Polyclonal Antibody
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Description
Polyclonal antibody to PAK6
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
PAK6 antibody; PAK5 antibody; p21 activated kinase 6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic Peptide of human PAK6
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:20 - 1:50
IF 1:20 - 1:50 -
Validations
Western blot - PAK6 Polyclonal Antibody
Western blot analysis of extracts of A-431 cells, using PAK6 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"