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Product Name
PAK4 antibody
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Description
PAK4 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human prostate cancer tissue. Positive FC detected in HeLa cells. Positive WB detected in COLO 320 cells, HeLa cells, PC-3 cells, rat skin tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 64 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1142 antibody; p21 activated kinase 4 antibody; PAK 4 antibody; PAK4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAK4 recombinant protein (Accession Number: XM_047438038). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:113500(PAK4 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113500(PAK4 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113500(PAK4 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using PAK4 antibody Catalog No:113500 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PAK4 (IP:Catalog No:113500, 4ug; Detection:Catalog No:113500 1:800) with mouse liver tissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug PAK4 antibody (Catalog No:113500, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).
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References
- Selamat W, Tay PL, Baskaran Y, Manser E. The Cdc42 Effector Kinase PAK4 Localizes to Cell-Cell Junctions and Contributes to Establishing Cell Polarity. PloS one. 10(6):e0129634. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"