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Product Name
PAFAH2 antibody
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Description
PAFAH2 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in mouse liver tissue, transfected cells, Transfected HEK-293 cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 44-46kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ26025 antibody; HSD PLA2 antibody; PAFAH2 antibody; SD PLA2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PAFAH2 recombinant protein (Accession Number: XM_006710670). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113488(PAFAH2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113488(PAFAH2 antibody) at dilution of 1:50 (under 10x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113488(PAFAH2 Antibody) at dilution of 1:800
IP Result of anti-PAFAH2 (IP:Catalog No:113488, 4ug; Detection:Catalog No:113488 1:300) with mouse liver tissue lysate 6400ug.
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Background
The family of platelet-activating factor-acetylhydrolase (PAF-AH) consists of enzymes which catalyze the hydrolysis of acetyl ester at the sn-2 position of PAF(Platelet-activating factor). The intracellular type II PAF-AH is a single polypeptide about a 40 kd distributed in both the cytosol and membranes of many kinds of cells.
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References
- Chen J, Yang L, Foulks JM, Weyrich AS, Marathe GK, McIntyre TM. Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis. Journal of lipid research. 48(11):2365-76. 2007.
- Thévenin AF, Monillas ES, Winget JM, Czymmek K, Bahnson BJ. Trafficking of platelet-activating factor acetylhydrolase type II in response to oxidative stress. Biochemistry. 50(39):8417-26. 2011.
- Latchoumycandane C, Marathe GK, Zhang R, McIntyre TM. Oxidatively truncated phospholipids are required agents of tumor necrosis factor α (TNFα)-induced apoptosis. The Journal of biological chemistry. 287(21):17693-705. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"