-
Product Name
PADI4 antibody
- Documents
-
Description
PADI4 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, Purified protein. Positive IP detected in mouse spleen tissue. Positive IF detected in A549 cells. Positive IHC detected in human tonsillitis tissue, human breast cancer tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 67 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
HL 60 PAD antibody; PAD antibody; PAD4 antibody; PADI4 antibody; PADI5 antibody; PDI4 antibody; PDI5 antibody; Peptidylarginine deiminase IV antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of PADI4 recombinant protein (Accession Number: NM_012387). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
WB result of PADI4 antibody (Catalog No:113483) with purified protein by T.J. Hollingsworth, Ph.D., University of Tennessee Health Sciences Center.
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:113483(PADI4 Antibody) at dilution of 1:600
IP Result of anti-PADI4 (IP:Catalog No:113483, 4ug; Detection:Catalog No:113483 1:500) with mouse spleen tissue lysate 4800ug.
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:113483(PADI4 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:113483(PADI4 Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using Catalog No:113483(PADI4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
PADI4, also named as PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues.(PMID: 19183436)
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"