Product Name

PADI2 antibody

Documents

Description

PADI2 Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells, mouse brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Positive IF detected in BxPC-3 cells, HeLa cells. Positive IHC detected in human gliomas tissue, human breast cancer tissue, human kidney tissue. Observed molecular weight by Western-blot: 49 kDa; 70-75 kDa

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

KIAA0994 antibody; PAD H19 antibody; PAD2 antibody; PADI2 antibody; PDI2 antibody; Peptidylarginine deiminase II antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of PADI2 recombinant protein (Accession Number: BC009701). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:1000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113482(PADI2 antibody) at dilution of 1:300

Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113482(PADI2 antibody) at dilution of 1:50 (under 10x lens)

IP Result of anti-PADI2 (IP:Catalog No:113482, 3ug; Detection:Catalog No:113482 1:300) with mouse brain tissue lysate 3600ug.

Immunofluorescent analysis of BxPC-3 cells using Catalog No:113482(PADI2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

References

McElwee JL, Mohanan S, Horibata S. PAD2 overexpression in transgenic mice promotes spontaneous skin neoplasia. Cancer research. 74(21):6306-17. 2014.
Arandjelovic S, McKenney KR, Leming SS, Mowen KA. ATP induces protein arginine deiminase 2-dependent citrullination in mast cells through the P2X7 purinergic receptor. Journal of immunology (Baltimore, Md. : 1950). 189(8):4112-22. 2012.
McElwee JL, Mohanan S, Griffith OL. Identification of PADI2 as a potential breast cancer biomarker and therapeutic target. BMC cancer. 12:500. 2012.
Zhang X, Bolt M, Guertin MJ. Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation. Proceedings of the National Academy of Sciences of the United States of America. 109(33):13331-6. 2012.
Mohanan S, Horibata S, McElwee JL, Dannenberg AJ, Coonrod SA. Identification of macrophage extracellular trap-like structures in mammary gland adipose tissue: a preliminary study. Frontiers in immunology. 4:67. 2013.
Guertin MJ, Zhang X, Anguish L. Targeted H3R26 deimination specifically facilitates estrogen receptor binding by modifying nucleosome structure. PLoS genetics. 10(9):e1004613. 2014.
Cherrington BD, Morency E, Struble AM, Coonrod SA, Wakshlag JJ. Potential role for peptidylarginine deiminase 2 (PAD2) in citrullination of canine mammary epithelial cell histones. PloS one. 5(7):e11768. 2010.
Zhang X, Gamble MJ, Stadler S. Genome-wide analysis reveals PADI4 cooperates with Elk-1 to activate c-Fos expression in breast cancer cells. PLoS genetics. 7(6):e1002112. 2011.

PADI2 antibody

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