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Product Name
PA2G4 antibody
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Description
PA2G4 Mouse Monoclonal antibody. Positive WB detected in Hela cells, HEK-293 cells, Jurkat cells, MCF7 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 38-42kD,48kD
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
EBP1 antibody; ErbB3 binding protein 1 antibody; HG4 1 antibody; p38 2G4 antibody; PA2G4 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of PA2G4 recombinant protein (Accession Number: NM_006191). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107448(PA2G4 antibody) at dilution of 1:1000
Immunofluorescent analysis of Hela cells, using PA2G4 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"