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Product Name
p75NTR antibody
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Description
p75NTR Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human brain tissue, human prostate hyperplasia tissue, human spleen tissue. Positive FC detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 70-75 kDa
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Tested applications
ELISA, IHC, IP, FC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CD271 antibody; Gp80 LNGFR antibody; NGF receptor antibody; NGFR antibody; p75 ICD antibody; p75(NTR) antibody; p75NTR antibody; TNFRSF16 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002507). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113561(NGFR Antibody) at dilution of 1:1000
IP Result of anti-NGFR (IP:Catalog No:113561, 3ug; Detection:Catalog No:113561 1:300) with mouse brain tissue lysate 5000ug.
1X10^6 SH-SY5Y cells were stained with 0.2ug NGFR antibody (Catalog No:113561, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical of paraffin-embedded human brain using Catalog No:113561(NGFR antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113561(NGFR antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:113561(NGFR Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NGFR, also named as TNFRSF16, Gp80-LNGFR, p75, ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.
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References
- Lu M, Dong J, Lu T. Effects of different sera conditions on olfactory ensheathing cells in vitro. International journal of molecular sciences. 16(1):420-38. 2015.
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