Cart 2

P53 antibody

Cat.#: 113553

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    P53 antibody
  • Documents
  • Description
    P53 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human endometrial cancer tissue, human kidney tissue, human lung cancer tissue, human lung tissue, human ovary tumor tissue, human placenta tissue, human skin tissue, human testis tissue. Positive WB detected in MCF-7 cells, A431 cells, MCF7 cells, mouse colon tissue, rat colon tissue. Positive IP detected in A431 cells. Observed molecular weight by Western-blot: 53 kDa
  • Tested applications
    ELISA, IHC, IP, WB, IF
  • Species reactivity
    Human, Rat, Mouse; other species not tested.
  • Alternative names
    Antigen NY CO 13 antibody; Cellular tumor antigen p53 antibody; FLJ92943 antibody; LFS1 antibody; P53 antibody; Phosphoprotein p53 antibody; TP53 antibody; TRP53 antibody; tumor protein p53 antibody; Tumor suppressor p53 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of P53 recombinant protein (Accession Number: NM_001126112). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    WB result of P53 antibody (Catalog No:113553, 1:2000) with si-p53 and si-Control transfected A431 cells.

    WB result of P53 antibody (Catalog No:113553, 1:2000) with si-p53 and si-Control transfected A431 cells.

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113553(P53 antibody) at dilution of 1:1000

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113553(P53 antibody) at dilution of 1:1000

    IP Result of anti-P53 (IP:Catalog No:113553, 4ug; Detection:Catalog No:113553 1:1000) with A431 cells lysate 3000ug.

    IP Result of anti-P53 (IP:Catalog No:113553, 4ug; Detection:Catalog No:113553 1:1000) with A431 cells lysate 3000ug.

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:113553(P53 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:113553(P53 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:113553(P53 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:113553(P53 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:113553(P53 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:113553(P53 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.
  • References
    • Yang CF, Peng LX, Huang TJ. Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute to radioresistance in nasopharyngeal carcinoma by suppressing the p53-mediated apoptosis pathway. Cancer letters. 344(2):260-71. 2014.
    • Mei L, Chen Y, Wang Z. Synergistic anti-tumour effects of tetrandrine and chloroquine combination therapy in human cancer: a potential antagonistic role for p21. British journal of pharmacology. 172(9):2232-45. 2015.
    • Yu L, Wang X, Zhu D. Disruption of human papillomavirus 16 E6 gene by clustered regularly interspaced short palindromic repeat/Cas system in human cervical cancer cells. OncoTargets and therapy. 8:37-44. 2015.
    • Zhang XF, Pan QZ, Pan K. Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma. Molecular carcinogenesis. 55(1):64-76. 2016.
    • Dan J, Sun X, Li W. 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Promotes Phenotypic Switching from Dedifferentiated to Differentiated Phenotype via Reactive Oxygen Species and p38 Mitogen-Activated Protein Kinase in Vascular Smooth Muscle Cells. Ultrasound in medicine & biology. 41(6):1681-9. 2015.
    • Dong H, Shi Q, Song X. Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. Toxicology and applied pharmacology. 286(1):10-6. 2015.
    • Sun S, Cheng B, Sun PG, Wu XH, Wu QQ, He P. RTEF-1 protects against oxidative damage induced by H2O2 in human umbilical vein endothelial cells through Klotho activation. Experimental biology and medicine (Maywood, N.J.). 240(12):1606-13. 2015.
    • Gu Y, He Y, Zhang X. Deficiency of monoclonal non-specific suppressor factor beta (MNSFB) promotes pregnancy loss in mice. Molecular reproduction and development. 82(6):475-88. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"