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Product Name
P53 antibody
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Description
P53 Mouse Monoclonal antibody. Positive WB detected in COLO 320 cells, A431 cells, HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 53 kDa
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Tested applications
ELISA, IP, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
Antigen NY CO 13 antibody; Cellular tumor antigen p53 antibody; FLJ92943 antibody; LFS1 antibody; Phosphoprotein p53 antibody; TRP53 antibody; tumor protein p53 antibody; Tumor suppressor p53 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of P53 recombinant protein (Accession Number: NM_001126112). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:50-1:500
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Validations
WB result of p53 antibody (Catalog No:107446, 1:1000) with sh-control and sh-p53 transfected A431 cells.
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107446(P53 Antibody) at dilution of 1:2000
IP Result of anti-P53 (IP:Catalog No:107446, 5ug; Detection:Catalog No:107446 1:1500) with HEK-293 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:107446(P53 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"