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Product Name
P4HTM Polyclonal Antibody
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Description
Polyclonal antibody to P4HTM
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
P4HTM antibody; EGLN4 antibody; HIFPH4 antibody; P4H-TM antibody; PH-4 antibody; PH4 antibody; PHD4 antibody; transmembrane prolyl 4-hydroxylase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 294-563 of human P4HTM (NP_808807.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - P4HTM Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using P4HTM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.
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Background
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"