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Product Name
P4HA3 antibody
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Description
P4HA3 Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive WB detected in mouse kidney tissue, A549 cells. Observed molecular weight by Western-blot: 30 kDa, 61 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
4 PH alpha 3 antibody; P4HA3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of P4HA3 recombinant protein (Accession Number: NM_182904). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:50-1:500
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113552(P4HA3 Antibody) at dilution of 1:300
Immunofluorescent analysis of (-20oc Ethanol) fixed A549 cells using Catalog No:113552(P4HA3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"