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P4HA1 antibody

Cat.#: 113550

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Special Price 239.0 USD

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Product Information

  • Product Name
    P4HA1 antibody
  • Documents
  • Description
    P4HA1 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human breast cancer tissue. Positive IP detected in A431 cells. Positive WB detected in A431 cells, HT-1080 cells, human heart tissue, rat skin tissue. Observed molecular weight by Western-blot: 61 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    4 PH alpha 1 antibody; P4HA antibody; P4HA1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of P4HA1 recombinant protein (Accession Number: NM_001142595). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113550(P4HA1 antibody) at dilution of 1:600

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113550(P4HA1 antibody) at dilution of 1:600

    IP Result of anti-P4HA1 (IP:Catalog No:113550, 3ug; Detection:Catalog No:113550 1:600) with A431 cells lysate 3300ug.

    IP Result of anti-P4HA1 (IP:Catalog No:113550, 3ug; Detection:Catalog No:113550 1:600) with A431 cells lysate 3300ug.

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113550(P4HA1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113550(P4HA1 antibody) at dilution of 1:100 (under 10x lens)

  • Background
    P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family ,which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites(uniprot). It can exsit as a heteromer, dimer or tetramer(GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61-63 kDa and 57-59 kDa.
  • References
    • Chakravarthi BV, Pathi SS, Goswami MT. The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression. Oncotarget. 5(16):6654-69. 2014.
    • Nagai M, Akita K, Yamada K, Okunishi I. The effect of isosaponarin isolated from wasabi leaf on collagen synthesis in human fibroblasts and its underlying mechanism. Journal of natural medicines. 64(3):305-12. 2010.
    • Pavlides S, Whitaker-Menezes D, Castello-Cros R. The reverse Warburg effect: aerobic glycolysis in cancer associated fibroblasts and the tumor stroma. Cell cycle (Georgetown, Tex.). 8(23):3984-4001. 2009.
    • Slany A, Haudek-Prinz V, Meshcheryakova A. Extracellular matrix remodeling by bone marrow fibroblast-like cells correlates with disease progression in multiple myeloma. Journal of proteome research. 13(2):844-54. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"