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Product Name
P4HA1 antibody
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Description
P4HA1 Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue, human testis tissue. Positive WB detected in PC-3 cells, HEK-293 cells. Observed molecular weight by Western-blot: 61 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
4 PH alpha 1 antibody; P4HA antibody; P4HA1 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of P4HA1 recombinant protein (Accession Number: NM_001142595). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:107444(P4HA1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107444(P4HA1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107444(P4HA1 Antibody) at dilution of 1:50
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Background
P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family ,which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites(uniprot). It can exsit as a heteromer, dimer or tetramer(GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.
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