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Product Name
P2RY2 Polyclonal Antibody
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Description
Polyclonal antibody to P2RY2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
P2RY2 antibody; HP2U antibody; P2RU1 antibody; P2U antibody; P2U1 antibody; P2UR antibody; P2Y2 antibody; P2Y2R antibody; P2Y purinoceptor 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 278-377 of human P2RY2 (NP_788086.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - P2RY2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using P2RY2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - P2RY2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using P2RY2 antibody . Blue: DAPI for nuclear staining.
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Background
Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"