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Product Name
P21 antibody
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Description
P21 Mouse Monoclonal antibody. Positive WB detected in MCF-7 cells, HEK-293 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse, Monkey; other species not tested.
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Alternative names
CAP20 antibody; CDK interacting protein 1 antibody; CDKN1 antibody; CDKN1A antibody; CIP1 antibody; MDA 6 antibody; MDA6 antibody; P21 antibody; p21CIP1 antibody; PIC1 antibody; SDI1 antibody; WAF1 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of P21 recombinant protein (Accession Number: NM_001220777). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107442(P21 Antibody) at dilution of 1:1000
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Background
CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
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References
- Imai R, Asai K, Hanai J, Takenaka M. Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation. Aging. 7(7):486-99. 2015.
- Guo X, Deng K, Wang H. GAS5 Inhibits Gastric Cancer Cell Proliferation Partly by Modulating CDK6. Oncology research and treatment. 38(7-8):362-6. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"