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Product Name
p19 INK4d antibody
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Description
p19 INK4d Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue. Positive WB detected in mouse testis tissue, mouse spleen tissue. Observed molecular weight by Western-blot: 19kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDKN2D antibody; INK4D antibody; P19 antibody; p19 INK4D antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of p19 INK4d recombinant protein (Accession Number: NM_079421). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113539(P19 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human testis using Catalog No:113539(P19 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:113539(P19 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CDKN2D antibody Catalog No:113539 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
CDKN2D represents cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
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References
- Sanda T, Li X, Gutierrez A. Interconnecting molecular pathways in the pathogenesis and drug sensitivity of T-cell acute lymphoblastic leukemia. Blood. 115(9):1735-45. 2010.
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