-
Product Name
p150 glued antibody
- Documents
-
Description
p150 glued Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, Jurkat cells. Positive IHC detected in human brain tissue. Positive IF detected in MDCK cells. Observed molecular weight by Western-blot: 135 kDa,150 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DAP 150 antibody; DCTN1 antibody; DP 150 antibody; Dynactin subunit 1 antibody; HMN7B antibody; P135 antibody; p150 glued antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004082). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113536(DCTN1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113536(DCTN1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113536(DCTN1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed MDCK cells using Catalog No:113536(p150 glued Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"