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Product Name
p115, USO1 antibody
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Description
p115, USO1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human gliomas tissue. Positive IP detected in mouse brain tissue. Positive WB detected in HepG2 cells, HEK-293 cells, human brain tissue, mouse testis tissue, mouse thymus tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 115kd
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
P115 antibody; Protein USO1 homolog antibody; TAP antibody; USO1 antibody; VDP antibody; Vesicle dOCking protein antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of p115, USO1 recombinant protein (Accession Number: NM_003715). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113532(p115, USO1 Antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113532(p115, USO1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using USO1 antibody Catalog No:113532 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-p115, USO1 (IP:Catalog No:113532, 3ug; Detection:Catalog No:113532 1:1500) with mouse brain tissue lysate 7000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113532(p115, USO1 antibody) at dilution of 1:100 (under 40x lens)
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Background
p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).
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References
- Kim S, Hill A, Warman ML, Smits P. Golgi disruption and early embryonic lethality in mice lacking USO1. PloS one. 7(11):e50530. 2012.
- Mojallal M, Zheng Y, Hultin S. AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion. Nature communications. 5:4557. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"