P1 peptide

P1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-AVPYPQ-OH

H-Ala-Val-Pro-Tyr-Pro-Gln-OH

6

95.01%

C₃₂H₄₇N₇O₉

673.75

Synthetic

Powder

P1 peptide(AVPYPQ) exhibited strong antioxidant potential against in vitro radicals with IC50 values for DPPH (5.12 ± 0.9-12.54 ± 0.6 µg/ml), ABTS (5.97 ± 0.2-14.20 ± 1.5 µg/ml), FRAP (4.98 ± 2.2-12.19 ± 0.8 µg/ml) and PSC (9.71 ± 0.5-17.84 ± 1.3 µg/ml),respectively. This peptide reduced ROS concentrations in Caco-2 cells treated with hydrogen peroxide. In silico studies indicated P1 had a higher binding score for the Keap1-Kelch domain than TX6, a potential Keap1 reference ligand.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Tyagi A, Chelliah R, Banan-Mwine Daliri E, Sultan G, Madar IH, Kim N, et al. Antioxidant activities of novel peptides from Limosilactobacillus reuteri fermented brown rice: A combined in vitro and in silico study. Food Chem. 2023;404(Pt B):134747

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.