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Product Name
P Glycoprotein Polyclonal Antibody
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Description
Polyclonal antibody to P Glycoprotein
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ABCB1 antibody; ABC20 antibody; CD243 antibody; CLCS antibody; GP170 antibody; MDR1 antibody; P-GP antibody; PGY1 antibody; multidrug resistance protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human P Glycoprotein (NP_000918.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - P Glycoprotein Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using P Glycoprotein antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - P Glycoprotein Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain endothelial cells using P Glycoprotein antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - P Glycoprotein Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using P Glycoprotein antibody at dilution of 1:100 (40x lens).
Immunofluorescence - P Glycoprotein Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using P Glycoprotein antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"