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Product Name
P-cadherin antibody
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Description
P-cadherin Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in A431 cells, K-562 cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in A431 cells. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cadherin 3 antibody; CDH3 antibody; CDHP antibody; HJMD antibody; PCAD antibody; P-cadherin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of P-cadherin recombinant protein (Accession Number: NM_001793). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113619(CDH3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113619(CDH3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113619(CDH3 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CDH3 (IP:Catalog No:113619, 4ug; Detection:Catalog No:113619 1:300) with A431 cells lysate 3040ug.
Immunofluorescent analysis of A431 cells using Catalog No:113619(CDH3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"