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Product Name
Oxytocin Receptor antibody
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Description
Oxytocin Receptor Rabbit Polyclonal antibody. Positive IP detected in L02 cells. Positive WB detected in Jurkat cells, L02 cells. Positive IHC detected in human hysteromyoma tissue, human breast cancer tissue. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
OT R antibody; OXTR antibody; OxytOCin Receptor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Oxyt℃in Receptor recombinant protein (Accession Number: NM_000916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113530(OXTR antibody) at dilution of 1:1000
IP Result of anti-OXTR (IP:Catalog No:113530, 4ug; Detection:Catalog No:113530 1:1000) with L02 cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human hysteromyoma tissue slide using Catalog No:113530(OXTR Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human hysteromyoma tissue slide using Catalog No:113530(OXTR Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 Jurkat cells were stained with 0.2ug OXTR antibody (Catalog No:113530, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). Oxytocin acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in endometrium, myometrium, mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR.
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References
- Elabd C, Cousin W, Upadhyayula P. Oxytocin is an age-specific circulating hormone that is necessary for muscle maintenance and regeneration. Nature communications. 5:4082. 2014.
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