-
Product Name
OXCT1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to OXCT1
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
OXCT1 antibody; OXCT antibody; SCOT antibody; 3-oxoacid CoA-transferase 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - OXCT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using OXCT1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Western blot - OXCT1 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and OXCT1 knockout HeLa cells, using OXCT1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - OXCT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using OXCT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - OXCT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using OXCT1 antibody at dilution of 1:100 (40x lens).
-
Background
Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"