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OTX2 antibody

Cat.#: 113519

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Product Information

  • Product Name
    OTX2 antibody
  • Documents
  • Description
    OTX2 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive FC detected in hESC cells. Positive IP detected in Y79 cells. Positive WB detected in Y79 cells. Observed molecular weight by Western-blot: 32kd
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Homeobox protein OTX2 antibody; MCOPS5 antibody; orthodenticle homeobox 2 antibody; Orthodenticle homolog 2 antibody; OTX2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of OTX2 recombinant protein (Accession Number: NM_021728). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:113519(OTX2 antibody) at dilution of 1:500

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:113519(OTX2 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113519(OTX2 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113519(OTX2 antibody) at dilution of 1:100 (under 40x lens)

    FC result of anti-OTX2 (Catalog No:113519, 1:100) with hESC which spontaneous differentiation toward retinal lineage (RPE). (Black: control; Blue OTX2).

    FC result of anti-OTX2 (Catalog No:113519, 1:100) with hESC which spontaneous differentiation toward retinal lineage (RPE). (Black: control; Blue OTX2).

    IP Result of anti-OTX2 (IP:Catalog No:113519, 3ug; Detection:Catalog No:113519 1:1500) with Y79 cells lysate 2400ug.

    IP Result of anti-OTX2 (IP:Catalog No:113519, 3ug; Detection:Catalog No:113519 1:1500) with Y79 cells lysate 2400ug.

  • Background
    The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive
  • References
    • Yang SH, Kalkan T, Morissroe C. Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency. Cell reports. 7(6):1968-81. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"