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Product Name
OTUD6B Polyclonal Antibody
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Description
Polyclonal antibody to OTUD6B
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
OTUD6B antibody; CGI-77 antibody; DUBA-5 antibody; DUBA5 antibody; IDDFSDA antibody; OTU domain containing 6B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 31-323 of human OTUD6B (NP_057107.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:200 -
Validations
Western blot - OTUD6B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using OTUD6B antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunoprecipitation - OTUD6B Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug OTUD6B antibody . Western blot was performed from the immunoprecipitate using OTUD6B antibody at a dilition of 1:1000.
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Background
Isoform 1: Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1. May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation. May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation. May also play a role in proteasome assembly and function.; Isoform 2: Stimulates protein synthesis. Influences the expression of CCND1/cyclin D1 by promoting its translation and regulates MYC/c-Myc protein stability.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"