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Product Name
OSGEP Polyclonal Antibody
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Description
Polyclonal antibody to OSGEP
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
OSGEP antibody; GCPL1 antibody; KAE1 antibody; OSGEP1 antibody; PRSMG1 antibody; TCS3 antibody; O-sialoglycoprotein endopeptidase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human OSGEP (NP_060277.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - OSGEP Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using OSGEP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - OSGEP Polyclonal Antibody
Immunofluorescence analysis of A549 cells using OSGEP antibody . Blue: DAPI for nuclear staining.
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Background
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"