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Product Name
OSBPL10 antibody
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Description
OSBPL10 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HEK-293 cells. Observed molecular weight by Western-blot: 75-80kd,64kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20363 antibody; ORP 10 antibody; ORP10 antibody; OSBP related protein 10 antibody; OSBP9 antibody; OSBPL10 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OSBPL10 recombinant protein (Accession Number: NM_017784). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113429(OSBPL10 antibody) at dilution of 1:400
IP result of anti-OSBPL10(Catalog No:113429 for IP and Detection).
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113429(OSBPL10 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113429(OSBPL10 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using OSBPL10 antibody Catalog No:113429 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease.
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References
- Nissilä E, Ohsaki Y, Weber-Boyvat M, Perttilä J, Ikonen E, Olkkonen VM. ORP10, a cholesterol binding protein associated with microtubules, regulates apolipoprotein B-100 secretion. Biochimica et biophysica acta. 1821(12):1472-84. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"