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Product Name
OSBP antibody
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Description
OSBP Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive WB detected in HeLa cells, human brain tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 89kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
OSBP antibody; OSBP1 antibody; oxysterol binding protein antibody; Oxysterol binding protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OSBP recombinant protein (Accession Number: NM_002556). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113428(OSBP antibody) at dilution of 1:800
IP result of anti-OSBP(Catalog No:113428 for IP and Detection).
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113428(OSBP antibody) at dilution of 1:50 (under 10x lens)
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Background
OSBP represents oxysterol binding protein
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References
- Dorobantu CM, Albulescu L, Harak C. Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus. PLoS pathogens. 11(9):e1005185. 2015.
- Wakana Y, Kotake R, Oyama N. CARTS biogenesis requires VAP-lipid transfer protein complexes functioning at the endoplasmic reticulum-Golgi interface. Molecular biology of the cell. 26(25):4686-99. 2015.
- Nhek S, Ngo M, Yang X. Regulation of oxysterol-binding protein Golgi localization through protein kinase D-mediated phosphorylation. Molecular biology of the cell. 21(13):2327-37. 2010.
- Auweter SD, Yu HB, Arena ET, Guttman JA, Finlay BB. Oxysterol-binding protein (OSBP) enhances replication of intracellular Salmonella and binds the Salmonella SPI-2 effector SseL via its N-terminus. Microbes and infection / Institut Pasteur. 14(2):148-54. 2012.
- Garbarino J, Pan M, Chin HF, Lund FW, Maxfield FR, Breslow JL. STARD4 knockdown in HepG2 cells disrupts cholesterol trafficking associated with the plasma membrane, ER, and ERC. Journal of lipid research. 53(12):2716-25. 2012.
- Wang H, Perry JW, Lauring AS, Neddermann P, De Francesco R, Tai AW. Oxysterol-binding protein is a phosphatidylinositol 4-kinase effector required for HCV replication membrane integrity and cholesterol trafficking. Gastroenterology. 146(5):1373-85.e1-11. 2014.
- Amini-Bavil-Olyaee S, Choi YJ, Lee JH. The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry. Cell host & microbe. 13(4):452-64. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"