Human Optineurin / OPTN (His & GST Tag) recombinant protein

Human Optineurin / OPTN (His & GST Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.

Optineurin

HUNTINGTIN-INTERACTING PROTEIN 7; ALS12; HUNTINGTIN YEAST PARTNER L; TFIIIA-INTP; NRP; HUNTINGTIN INTERACTING PROTEIN L; FIP2; OPTIC NEUROPATHY-INDUCING PROTEIN; E3-14.7K-INTERACTING PROTEIN; TRANSCRPTION FACTOR IIIA-INTERACTING PROTEIN; TRANSCRIPTION FACTOR IIIA-INTERACTING PROTEIN; FIP-2; HYPL; RP23-379M5.2; 4930441O07RIK; NEMO-RELATED PROTEIN; HIP7; TUMOR NECROSIS FACTOR ALPHA-INDUCIBLE CELLULAR PROTEIN CONTAINING LEUCINE ZIPPER DOMAINS; OPTINEURIN; HIP-7; RP11-730A19.1; OPTN; GLC1E

Q96CV9

OPTN; FIP2; GLC1E; HIP7; HYPL; NRP

Baculovirus-Insect Cells

A DNA sequence encoding the human OPTN (NP_068815.2) (Met1-Ile577) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human OPTN chimera consists of 814 amino acids and has a calculated molecular mass of 93.7 kDa. The recombinant protein migrates approximately 94 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE