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Product Name
OLIG2 antibody
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Description
OLIG2 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Positive WB detected in human brain tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BHLHB1 antibody; bHLHe19 antibody; OLIG2 antibody; OLIGO2 antibody; PRKCBP2 antibody; RACK17 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OLIG2 recombinant protein (Accession Number: XM_005260908). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113341(OLIG2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113341(OLIG2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using Catalog No:113341 and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-OLIG2 (IP:Catalog No:113341, 3ug; Detection:Catalog No:113341 1:500) with mouse brain tissue lysate 4000ug.
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Background
OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )
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References
- Du M, Butchi NB, Woods T, Morgan TW, Peterson KE. Neuropeptide Y has a protective role during murine retrovirus-induced neurological disease. Journal of virology. 84(21):11076-88. 2010.
- Ge H, Tan L, Wu P. Poly-L-ornithine promotes preferred differentiation of neural stem/progenitor cells via ERK signalling pathway. Scientific reports. 5:15535. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"