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Product Name
OLFM3 antibody
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Description
OLFM3 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, A2780 cells, human liver tissue, NIH/3T3 cells, PC-3 cells. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
NOE3 antibody; Noelin 3 antibody; NOELIN3 antibody; NOELIN3_V1 antibody; NOELIN3_V2 antibody; NOELIN3_V3 antibody; NOELIN3_V4 antibody; NOELIN3_V5 antibody; NOELIN3_V6 antibody; olfactomedin 3 antibody; OLFM3 antibody; OPTIMEDIN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OLFM3 recombinant protein (Accession Number: NM_058170). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113340(OLFM3 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using OLFM3 antibody Catalog No:113340 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:113340(OLFM3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:113340(OLFM3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
OLFM3 represents olfactomedin 3
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"