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Product Name
OIP5 antibody
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Description
OIP5 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, HeLa cells, PC-3 cells. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
5730547N13Rik antibody; Cancer/testis antigen 86 antibody; CT86 antibody; LINT 25 antibody; MIS18B antibody; MIS18beta antibody; OIP 5 antibody; OIP5 antibody; Opa interacting protein 5 antibody; Protein Mis18 beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OIP5 recombinant protein (Accession Number: NM_007280). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113337(OIP5 antibody) at dilution of 1:500
IP Result of anti-OIP5 (IP:Catalog No:113337, 3ug; Detection:Catalog No:113337 1:500) with Jurkat cells lysate 3200ug.
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Background
OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.
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References
- Wang J, Liu X, Dou Z. Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP). The Journal of biological chemistry. 289(12):8326-36. 2014.
- Yuan Q, Han J, Cong W. Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity. International journal of nanomedicine. 9:4829-46. 2014.
- Koinuma J, Akiyama H, Fujita M. Characterization of an Opa interacting protein 5 involved in lung and esophageal carcinogenesis. Cancer science. 103(3):577-86. 2012.
- Gong M, Xu Y, Dong W. Expression of Opa interacting protein 5 (OIP5) is associated with tumor stage and prognosis of clear cell renal cell carcinoma. Acta histochemica. 115(8):810-5. 2013.
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