Product Name

OGDH antibody

Documents

Description

OGDH Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human lung cancer tissue. Positive WB detected in mouse lung tissue, A549 cells, mouse heart tissue, mouse liver tissue, mouse pancreas tissue, rat heart tissue, rat lung tissue, ROS1728 cells. Observed molecular weight by Western-blot: 116kd

Tested applications

ELISA, IHC, IF, WB

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

AKGDH antibody; E1k antibody; OGDC antibody; OGDC E1 antibody; OGDH antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of OGDH recombinant protein (Accession Number: NM_002541). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113331(OGDH antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113331(OGDH antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of Hela cells, using OGDH antibody Catalog No:113331 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:113331(OGDH Antibody) at dilution of 1:3000

Background

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.

References

Chen L, Shu Y, Liang X. OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin. Proceedings of the National Academy of Sciences of the United States of America. 111(27):9983-8. 2014.
Yao J, Chen S, Mao Z, Cadenas E, Brinton RD. 2-Deoxy-D-glucose treatment induces ketogenesis, sustains mitochondrial function, and reduces pathology in female mouse model of Alzheimer's disease. PloS one. 6(7):e21788. 2011.
Yao J, Zhao L, Mao Z. Potentiation of brain mitochondrial function by S-equol and R/S-equol estrogen receptor β-selective phytoSERM treatments. Brain research. 1514:128-41. 2013.
Jiang T, Yin F, Yao J, Brinton RD, Cadenas E. Lipoic acid restores age-associated impairment of brain energy metabolism through the modulation of Akt/JNK signaling and PGC1α transcriptional pathway. Aging cell. 12(6):1021-31. 2013.

OGDH antibody

Product Information

Related Products / Services