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Product Name
OGDH antibody
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Description
OGDH Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue. Positive WB detected in human heart tissue, ROS1728 cells. Observed molecular weight by Western-blot: 116 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
AKGDH antibody; E1k antibody; OGDC antibody; OGDC E1 antibody; OGDH antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of OGDH recombinant protein (Accession Number: NM_002541). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:50-1:500
IF: 1:50-1:500
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107345(OGDH Antibody) at dilution of 1:2500
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:107345(OGDH Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:107345(OGDH Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:107345(OGDH Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
OGDH represents oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
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