Odorranain-B1 peptide

Odorranain-B1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

AALKGCWTKSIPPKPCFGKR

Ala-Ala-Leu-Lys-Gly-Cys-Trp-Thr-Lys-Ser-Ile-Pro-Pro-Lys-Pro-Cys-Phe-Gly-Lys-Arg

20

97.7%

Synthetic

Powder

The peptide has been reported in different articles in different names. In addition to antimicrobial activity, the peptide also inhibits trypsin due to the disulfide-bonded trypsin inhibitory loop in the sequence (enzyme inhibitor) (Li J et al 2007 FASEB J 21: 2466-73). In this proteomic paper, 372 cDNA sequences of antimicrobial peptides were found in a single frog. Among them, 107 are new peptides. Approximately 40 peptides were synthesized and tested. Only 24 peptides with antimicrobial acitivities (APD entries 1291-1314) were registered into this database. OBR1 is active against E. coli ATCC25922 (MIC 5.83 ug/ml), S. aureus ATCC2592 (MIC 5.83 ug/ml), B. dysenteria (1.84 ug/ml), and C. albicans ATCC2002 (2.4 ug/ml). Derivatives: ORB-C (sequence CWTKSIPPKPC, disulfide bridged) has improved trypsin inhibition ability but lost its antimicrobial activity. ORB1 is N-terminal AA truncated form. Odorranain-B1 has Antibacterial, Antifungal activity. The source of Odorranain-B1 is Odorrana grahami, Asia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Li J, Xu X, Xu C, et al. Anti-infection peptidomics of amphibian skin. Mol Cell Proteomics. 2007;6(5):882-94.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.