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Product Name
OCTN2 antibody
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Description
OCTN2 Rabbit Polyclonal antibody. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in mouse small intestine tissue, human placenta tissue, mouse pancreas tissue, mouse skeletal muscle tissue. Positive IHC detected in human kidney tissue. Positive FC detected in HEK-293 cells. Observed molecular weight by Western-blot: 70-80 kDa
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Tested applications
ELISA, WB, IHC, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDSP antibody; FLJ46769 antibody; OCTN2 antibody; OCTN2VT antibody; SLC22A5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ℃TN2 recombinant protein (Accession Number: NM_003060). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:113320(SLC22A5 antibody) at dilution of 1:500
IP Result of anti-SLC22A5 (IP:Catalog No:113320, 4ug; Detection:Catalog No:113320 1:500) with mouse skeletal muscle tissue lysate 2200ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113320(SLC22A5 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113320(SLC22A5 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HEK-293 cells were stained with 0.2ug SLC22A5 antibody (Catalog No:113320, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ocTN2 (Organic Cation/Carnitine Transporter 2), encoded by SLC22A5, is a ubiquitously expressed organic anion/cation transporter. ocTN2 plays a key role in absorption, tissue distribution and renal reabsorption of L-carnitine which is an essential cofactor in fat metabolism. Defects in the ocTN2 are responsible for primary carnitine deficiency. ocTN2 also transports some important respiratory medicines (such as ipratropium and tiotropium), and due to its expression in the lung, may influence the disposition and absorption of these medicines in the lung.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"