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Product Name
OBFC2A antibody
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Description
OBFC2A Rabbit Polyclonal antibody. Positive WB detected in COLO 320 cells. Observed molecular weight by Western-blot: 22kd
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Tested applications
ELISA, WB
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
OBFC2A antibody; DKFZp667M1322 antibody; hSSB2 antibody; SSB2 antibody; Sensor of ssDNA subunit B2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OBFC2A recombinant protein (Accession Number: NM_001254736). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:113463(OBFC2A antibody) at dilution of 1:500
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Background
Single-stranded DNA (ssDNA)-binding proteins (SSBs) are ubiquitous and essential for a variety of DNA metabolic processes, including recombination, replication, and detection and repair of damage. SSBs have multiple roles in binding and sequestering ssDNA, detecting DNA damage, stimulating nucleases, helicases and strand-exchange proteins, activating transcription and mediating protein-protein interactions [PMID:18449195] Human single-stranded DNA-binding protein 1 (hSSB1), encoded by OBFC2B, is characterized as an essential factor for the initiation of DNA damage checkpoints and the maintenance of genomic stability [PMID:22940690].
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References
- Feldhahn N, Ferretti E, Robbiani DF. The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo. The EMBO journal. 31(20):4045-56. 2012.
- Skaar JR, Ferris AL, Wu X. The Integrator complex controls the termination of transcription at diverse classes of gene targets. Cell research. 25(3):288-305. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"