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Product Name
NWD1 antibody
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Description
NWD1 Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, HepG2 cells, mouse brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 77 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
NWD1 antibody; NWD1 Ens antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NWD1 recombinant protein (Accession Number: NM_001347994). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:113450(NWD1 Antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells using Catalog No:113450(NWD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NWD1 also named as NWD1 Ens or NACHT and WD repeat domain containing 1 is a 1564 amino acid protein, which contains 1 NACHT domain and 13 WD repeat. The molecular weight of NWD1 was detected by western blot. The result shows that the molecular weight of NWD1 is 77 kDa., but NWD1 is a 174 kDa protein according to UniProt,. The function of NWD1 is still non-known, meanwhile the molecular weight of NWD1 is still remained to be confirmed.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"