-
Product Name
NUP98 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to NUP98
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse
-
Alternative names
NUP98 antibody; ADIR2 antibody; NUP196 antibody; NUP96 antibody; nucleoporin 98 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 621-920 of human NUP98 (NP_624357.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:1000 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NUP98 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NUP98 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - NUP98 Polyclonal Antibody
Immunofluorescence analysis of L929 cells using NUP98 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"