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Product Name
NUP62 Polyclonal Antibody
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Description
Polyclonal antibody to NUP62
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
NUP62 antibody; IBSN antibody; SNDI antibody; p62 antibody; nucleoporin 62 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 403-522 of human NUP62 (NP_036478.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - NUP62 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NUP62 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunohistochemistry - NUP62 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using NUP62 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - NUP62 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NUP62 antibody . Blue: DAPI for nuclear staining.
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Background
Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"