-
Product Name
NUP133 antibody
- Documents
-
Description
NUP133 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 129kd
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
133 kDa nucleoporin antibody; FLJ10814 antibody; hNUP133 antibody; nucleoporin 133kDa antibody; Nucleoporin Nup133 antibody; NUP133 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of NUP133 recombinant protein (Accession Number: NM_018230). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113374(NUP133 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113374(NUP133 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-NUP133 (IP:Catalog No:113374, 4ug; Detection:Catalog No:113374 1:800) with HeLa cells lysate 880ug.
-
Background
Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"